Mar 23 2021

A UNIQUE IN-VIVO MODEL FOR FAST ADC EFFICACY RANKING

 

Poster abstract

 

Since its introduction, xenografts on the chicken embryo’s ChorioAllantoic Membrane (CAM) has proven extremely valuable for in vivo studies of tumor development, angiogenesis and malignant cell dissemination. The CAM’s ability to efficiently grow inoculated xenogenic tumor cells greatly simplify the analysis of human tumor cell metastasis. Here we demonstrate that our in ovo model is useful for rapidly testing and comparing efficacy of Antibody Drug Conjugates (ADCs) not only on tumors, but also on metastasic invasion.
Embryonated chicken eggs N87 gastric carcinoma cells grafted on the CAM were treated with different ADCs consisting of trastuzumab (Herceptin®) and 5 different payloads.
Tumor efficacy was measured via the reduction in tumor weight compared to the negative control group (treated with only the vehicle). Thus, we observed a dose-dependent effect for all ADCs and were able to rank them according to their efficacy from 0% (Ctrl ADC) to 60% reduction in tumor weight for the most effective.
In addition to efficacy on primary tumors, we also determined the effect of ADCs on the dissemination of cancer cells. Thus, two ADCs with the same efficacy on tumors showed two radically different effects on metastasis: one showed no particular efficacy while the second reduced metastasis to 20% of those observed in the negative control group.

 


 

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